Bra phenotype of bra5-2, bra2-3, and bra4-2 mutant strains is

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Download scientific diagram | Bra phenotype of bra5-2, bra2-3, and bra4-2 mutant strains is complemented by ADE13. Yeast strains 206 (bra5-2), 211 (bra2-3), 242 (bra4-2), and the isogenic wild-type PLY122 were cotransformed with a plasmid carrying the ADE1-lacZ fusion (P473) and a plasmid carrying the ADE13 gene (P1813) or the corresponding empty centromeric vector (pFL38, lanes ). Gal assays were performed after growth for 6 h in SC medium in the absence (low ade) or presence (high ade) of adenine (0.3 mM), as described in Materials and Methods. from publication: Yeast AMP Pathway Genes Respond to Adenine through Regulated Synthesis of a Metabolic Intermediate | In Saccharomyces cerevisiae, AMP biosynthesis genes (ADE genes) are transcriptionally activated in the absence of extracellular purines by the Bas1p and Bas2p (Pho2p) transcription factors. We now show that expression of theADE genes is low in mutant strains affected in the | Adenine, Yeasts and Two-Hybrid System Techniques | ResearchGate, the professional network for scientists.